A defined molecular-weight distribution of deoxyribonucleic acid after extensive sonication

A defined molecular-weight distribution of deoxyribonucleic acid after extensive sonication.

Concentrated solutions of low-molecular-weight DNA (Mw=35000) with a known molecular-weight distribution can be prepared in several hours, and require no additional fractionation procedures. This is achieved by sonication of the DNA in 1.0 M-NaCl at high power at 0--2 degrees C. No denaturation of the DNA is detectable, even after 8h of continuous sonication. After 2h, the molecular-weight dist...

Molecular weight of bacteriophage PBS 1 deoxyribonucleic acid.

Molecular Structure of Deoxyribonucleic Acid (dna)

Although DNA is associated with complex biological phenomena and has many physicochemical properties not easily explained, there is no doubt that the molecule consists to a large extent of the regular, and in some ways simple structure (1-3), derived from x-ray diffraction. We will describe here briefly new data on the s t ruc turethe x-ray diffraction measurements and molecular model building ...

Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme.

Replication of polydeoxynucleotide templates catalyzed by the low molecular weight DNA polymerase from calf thymus chromatin proceeds only in the presence of a complementary initiator, complementary deoxynucleoside triphosphate, and a divalent cation. The minimum chain length of an initiator for initiation of polydeoxyadenylate is 7. Kinetic studies show a single binding site for deoxynucleosid...

Rheological Method for Determining Molecular Weight and Molecular Weight Distribution